TOYOPEARL HW-75F
Brand: TOYOPEARL
Manufacturers:
Description
TOYOPEARL HW-75F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-75F resin has a pore size of > 100 nm and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-75F resin useful for protein separations of biomolecules between 500,000 and 50,000,000 Da in size.
The good mechanical stability of the TOYOPEARL HW-75F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-75F resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.
If higher resolution is needed, the smaller particle TOYOPEARL HW-75S resin can also be used for process SEC.
Specifications
| Product Category | Process Media |
|---|---|
| Particle Size (µm) | 45 |
| Process Step | Polishing |
| Mode | Size Exclusion |
| Resin Volume | 500 mL, 150 mL, 1 L, 5 L |
| Applications | Proteins, Peptides, Enzymes, Plasma purification, Vaccines, Aggregates, Other recombinant proteins, Virus |
| Group | Specialty |
| Pore Size (nm) | 101 |