TOYOPEARL SP-650M
Brand: TOYOPEARL
Manufacturers:
Description
TOYOPEARL SP-650M media is a strong cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that has been functionalized with sulfopropyl (S) strong cation exchange groups. TOYOPEARL SP-650M resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL SP-650M resin has a 100 nm pore size and a 65 µm bead size.
TOYOPEARL SP-650M resin is best used for high throughput intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle TOYOPEARL SP-650S or TSKgel SP-5PW resins may be used. As both the TOYOPEARL SP-650 and TSKgel SP-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.
TOYOPEARL SP-650M has a dynamic binding capacity for lysozyme of 50 g/L. TOYOPEARL SP-650M resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.
Specifications
| Resin Volume | 1 L, 5 L, 100 mL, 50 L, 250 mL |
|---|---|
| Product Category | Process Media |
| Process Step | Intermediate Purification |
| Applications | Aggregates, Lipoproteins, Monoclonal antibodies, mAb fragments, Peptides, Enzymes, Proteins, Other recombinant proteins |
| Mode | Cation Exchange |
| Group | Specialty |
| Particle Size (µm) | 65 |
| Pore Size (nm) | 100 |