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Spotlight

Anion Exchange - Columns

Very efficient chromatography can be achieved with the nonporous TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. Applications for the TSKgel Q-STAT columns include the separation of: proteins, peptides, low molecular weight nucleic acids, aggregates and charge isomers of monoclonal antibodies, PEGylated proteins, oligo DNA, and siRNA. The TSKgel DNA-STAT column is ideal for the analysis of DNA fragments, nucleic acids and nucleotides.

The pore structure and bonding chemistry of TSKgel BioAssist Q columns provides high capacity for small to very large molar mass proteins and nucleic acids.

Proteins, peptides, DNA and RNA-derived oligonucleotides, and other nucleic acid fragments are typical samples that are analyzed or isolated on the TSKgel DEAE-5PW and TSKgel Super Q-5PW columns.

TSKgel DEAE-NPR nonporous columns are used for the high speed separation and analysis of proteins and poly and oligonucleotides. Applications for the TSKgel DNA-NPR nonporous columns include the high efficiency separation of DNA fragments, PCR products, or plasmids.

TSKgel DEAE-2SW and TSKgel QAE-2SW (both 5 µm, 12.5 nm) and TSKgel DEAE-3SW (10 µm, 25 nm) are available for analyzing smaller molar mass samples such as nucleotides, drug candidates, catecholamines, and small peptides or proteins.

TSKgel Sugar AXG columns are packed with 10 µm beads and are used for the gradient separation and analysis of monosaccharides, disaccharides, and sugar alcohols.  
For isocratic separation of carbohydrates where lower and constant back pressures may be generated, the 8 µm TSKgel Sugar AXI column is optimal.

TSKgel SAX columns are used for the separation of isomerized sugars, alcohols, and low molar mass organic acids.
 

TSKgel BioAssist Q

Base Material:  polymethacrylate  
Particle Size (mean):  10 µm and 13 µm
Pore Size (mean):  400 nm
Functional Group:  polyamine
Counter Ion:  CI-  
pH Range:  2.0 - 12.0  
Capacity (mg BSA/mL):  70
Small Ion Capacity:  0.1 meq/mL  
pKa:  9.4  

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TSKgel DEAE-2SW

Base Material:  hydrophilic silica  
Particle Size (mean):  5 µm  
Pore Size (mean):  12.5 nm
Functional Group:  CH2CH2N+(C2H5)3  
Counter Ion:  H2PO4-  
pH Range:  2.0 - 7.5  
Capacity (mg BSA/mL):  ND
Small Ion Capacity:  > 0.3 meq/mL  
pKa:  11.2  

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TSKgel DEAE-3SW

Base Material:  hydrophilic silica  
Particle Size (mean): 10 µm  
Pore Size (mean):  25 nm
Functional Group:  CH2CH2N+(C2H5)3  
Counter Ion:  CI-
pH Range:  2.0 - 7.5  
Capacity  (mg BSA/mL):  ND
Small Ion Capacity:  > 0.3 meq/mL  
pKa:  11.2  

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TSKgel DEAE-5PW

Base Material:  hydroxylated methacrylate polymer  
Particle Size (mean):  10, 13 and 20 µm  
Pore Size (mean):  100 nm
Functional Group:  CH2CH2N+(C2H5)3  
Counter Ion:  CI-  
pH Range:  2.0 - 12.0  
Capacity (mg BSA/mL):  30
Small Ion Capacity:  > 0.1 meq/mL  
pKa:  11.5  

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TSKgel DEAE-NPR

Base Material:  hydroxylated methacrylate polymer  
Particle Size (mean):  2.5 µm 
Pore Size (mean):  non-porous
Functional Group:  CH2CH2N+(C2H5)3  
Counter Ion:  CI-  
pH Range: 
 
2.0 - 12.0 
 
Capacity  (mg BSA/mL):  5
Small Ion Capacity:  > 0.1 meq/mL  
pKa:  11.2  

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TSKgel DNA-NPR

Base Material:  hydroxylated methacrylate polymer  
Particle Size (mean):  2.5 µm  
Pore Size (mean):  non-porous
Functional Group:  proprietary
Counter Ion:  CIO4-  
pH Range:  2.0 - 12.0  
Capacity (mg BSA/mL):  5
Small Ion Capacity:  > 0.1 meq/mL  
pKa:  11.2  

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TSKgel DNA-STAT

Base Material:  hydrophilic polymer  
Particle Size (mean):  5 µm  
Pore Size (mean):  non-porous
Functional Group:  quarternary ammonium  
Counter Ion:  CI  
pH Range:  3.0 - 10.0  
Static Binding Capacity  (mg BSA/g dry gel):  ca. 35 (5 µm)
Small Ion Capacity:  270 µeg/g dry gel  
pKa:  10.5  
Hardware:  Stainless Steel tubing & fittings, PEEK frits  

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TSKgel QAE-2SW

Base Material:  silica  
Particle Size (mean):  5 µm  
Pore Size (mean):  12.5 nm
Functional Group:  trimethylamino  
Counter Ion:  H2PO4-  
pH Range:  2.0 - 7.5  
Capacity (mg BSA/g dry gel):  ND
Small Ion Capacity:  > 0.3 meq/mL  
pKa:  11.2  

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TSKgel Q-STAT

Base Material:  hydrophilic polymer  
Particle Size (mean):  7 µm and 10 µm  
Pore Size (mean):  non-porous
Functional Group:  quarternary ammonium  
Counter Ion:  CI
pH Range:  3.0 - 10.0  
Static Binding Capacity  (mg BSA/g dry gel):  ca. 35 (7 µm) ca. 20 (10 µm)  
Small Ion Capacity:  270 µeg/g dry gel  
pKa:  10.5  
Hardware:  Stainless Steel tubing & fittings, PEEK frits   

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TSKgel SAX

Base Material:  PS-DVB polymer  
Particle Size (mean):  5 µm  
Pore Size (mean):  6.0 nm
Functional Group:  trimethylamine
Counter Ion:  CI-
pH Range: 1.0 - 14.0  
Capacity (mg BSA/mL): ND
Small Ion Capacity: >0.1 meq/mL
pKa: 12.5  

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TSKgel Sugar AXG

Base Material:PS-DVB ploymer
Particle Size (mean):10 µm 
Pore Size (mean):6.0 nm
Functional Group:trimethylamino
Counter Ion:HBO3-
pH Range:1.0 - 14.0
Capacity (mg BSA/mL):NA
Small Ion Capacity:> 1.2 meq/mL
pKa:12.5

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TSKgel Sugar AXI

Base Material:PS-DVB polymer
Particle Size (mean):8 µm
Pore Size (mean):6.0 nm
Functional Group:trimethylamino
Counter Ion:HBO3-
pH Range:1.0 - 14.0
Capacity (mg BSA/mL):ND
Small Ion Capacity:> 1.2 meq/mL
pKa:12.5

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TSKgel SuperQ-5PW

Base Material:polymethacrylate
Particle size (mean):10 µm and 13 µm
Pore size (mean):100 nm
Functional group:trymethylamino
Counter Ion:CI-
pH stability:2.0 - 12.0
Capacity (mg BSA/mL):100
Small Ion Capacity:> 0.13 meq/mL
pKa:12.2

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