Analysis of biomolecules by native (U)HPLC Workshop
Native HPLC and Size Exclusion for Bioanalysis: Courses on HIC, IEX, SEC and MALS are offered three times a year and can be booked individually.
Who should attend?
Anyone involved in the development, analysis, or quality control of biomolecules who wants to gain practical insights into modern (U)HPLC techniques.
What will it be about?
From proteins and oligonucleotides to complex biotherapeutics, this course provides an overview of key (U)HPLC methods and demonstrates how they can be used to address real-world analytical challenges in biomolecule characterization.
Day
Time
Presentations
Tue Morning HIC
9:00
Opening Remarks
9:30
Fundamentals of HIC
10:00
Method development (column, mobile phase etc.)
10:45
Coffee Break
11:00
Applications and case studies
11:40
Troubleshooting (& discussion of your own chromatograms)
12:15
Lunch
Tue Afternoon IEX
13:15
Fundamentals of IEX
13:45
Method development (column, mobile phase etc.)
14:30
Coffee Break
14:45
Applications and case studies
15:30
Troubleshooting (& discussion of your own chromatograms)
16:30
End of day
Wed SEC
9:30
Basics of SEC
10:00
Column selection, Column calibration
11:15
Coffee Break
11:30
Mobile phase selection
12:15
Lunch
13:15
Detectors
14:00
Applications for Biomolecules
14:45
Coffee Break
15:00
Troubleshooting (& discussion of your own chromatograms)
18:00
Dinner & Outing
Thu MALS
9:30
Introduction to MALS
10:30
MALS in combination with other detectors (incl. triple detection)