TOYOPEARL HW-50F
Brand: TOYOPEARL
Manufacturers:
Description
TOYOPEARL HW-50F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-50F resin has a 12.5 nm pore size and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-50F resin useful for protein separations of biomolecules between 500 and 80,000 Da in size.
The good mechanical stability of the TOYOPEARL HW-50F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-50F resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.
If higher resolution is needed, the smaller particle TOYOPEARL HW-50S resin can also be used for process SEC.
Specifications
| Resin Volume | 50 L, 500 mL, 1 L, 150 mL, 5 L |
|---|---|
| Product Category | Process Media |
| Pore Size (nm) | 12.5 |
| Particle Size (µm) | 45 |
| Process Step | Polishing |
| Mode | Size Exclusion |
| Applications | Enzymes, Vaccines, Peptides, Other recombinant proteins |
| Group | Specialty |