
Brand: TOYOPEARL
Manufacturers:
TOYOPEARL HW-65S resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-65S resin has a 100 nm pore size and a 30 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-65S resin useful for protein separations of biomolecules between 40,000 and 5,000,000 Da in size.
The good mechanical stability of the TOYOPEARL HW-65S resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-65S resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.
If lower resolution is needed, the larger particle TOYOPEARL HW-65F resin can also be used for process SEC.
| Resin Volume | 150 mL, 1 L, 5 L, 250 mL, 50 L, 500 mL |
|---|---|
| Product Category | Process Media |
| Process Step | Polishing |
| Mode | Size Exclusion |
| Particle Size (µm) | 30 |
| Applications | Other recombinant proteins, Virus, Enzymes, Proteins, Peptides, Aggregates, Plasma purification, Vaccines |
| Group | Specialty |
| Pore Size (nm) | 100 |