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Affinity Chromatography

  Affinity Chromatography (AFC) makes use of specific binding interactions between molecules. This specificity makes it attractive as a capture step to isolate the target molecule from crude extracts. A particular ligand is chemically immo...
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Gel permeation chromatography (organic SEC)

TSKgel Gel Permeation Chromatography ColumnsGel Permeation Chromatography (GPC) plays an important role in the characterization of polar organic-soluble and organic-soluble polymers in consumer, chemical and petrochemical industries.TSKgel Alpha and ...
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Gel filtration chromatography (aqueous SEC)

TSKgel Gel Filtration Chromatography ColumnsGel Filtration Chromatography (GFC) is popular among biochemists for the separation of proteins or nucleic acid fractions, to desalt a protein sample, or to characterize water-soluble polymers used in food ...
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EcoSEC High Temperature GPC System

EcoSEC High Temperature SystemDemanding high temperature polymer analyses, such as for polyolefins, require a system that delivers results reliably, reproducibly, and produces these results in an easy to use, safe instrument. The EcoSEC High Temperat...
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HIC columns – what they do and how to find the right one

HIC columns – hydrophobic interaction chromatography for (U)HPLC analysisHydrophobic interaction chromatography (HIC) is a technique that separates molecule mixtures according to their hydrophobicity. It is popular for analyzing and purifying protein...
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Cation Exchange

TSKgel Cation Exchange Chromatography ColumnsVery efficient chromatography can be achieved with the nonporous TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. Applications for the TSKgel CM-STAT and TSKgel SP-STA...
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Lens3 MALS Detector

LenS3 Multi-Angle Light Scattering DetectorsThe next generation of LenS™3 MALS detector provides the latest in light scattering detection from Tosoh Bioscience. Technological advancements in instrument design and software simplify exact molecular wei...
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Products

SolutionsThis Solutions section is designed to help you learn about the theory and practice of liquid chromatography to aid you in selecting the best Tosoh product for your application. The section is organized by product group and contains an overvi...
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Chromatography instruments

As an expert in chromatography solutions, Tosoh Bioscience offers chromatography equipment that allows you to achieve the best results for your chromatographic applications.GPC instruments – all-in-one solution for polymer analysisEcoSEC is a compact...
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Process Media for Purification

Process ChromatographyChromatography is a common purification step in industrial downstream processing (DSP) of biological targets. A typical DSP scheme combines two or more chromatographic separation steps with ultra- and diafiltration unit operatio...
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Hydrophilic Interaction Chromatography - Columns

Hydrophilic Interaction ChromatographyNormal phase and hydrophilic interaction liquid chromatography (HILIC) are primarily used to separate polar and hydrophilic compounds. In reversed phase mode very polar compounds are often not sufficiently retain...
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Anion Exchange - Columns

Very efficient chromatography can be achieved with the nonporous TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. Applications for the TSKgel Q-STAT columns include the separation of: proteins, peptides, low mole...
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MCC Applications

The Octave TM systems can be used for a variety of applications, including:Intensified chromatography unit operationIntegrated continuous processingParallel batch chromatographySimulated moving bedList of MCC ApplicationsYearTitleAuthors  Journa...
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Benefits of MCC

Multi-column chromatography results in higher productivity due to higher load levels, faster flowrates, and parallelization of non-loading steps.Multi-column chromatography provides the following benefitsReduced resin consumption (cost savings)Increa...
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Holistic Multi-Column Chromatography Solution

Multi-column chromatography is a liquid chromatographic technique used to purify solutions via a cyclic usage of multiple columns that is faster and more cost-effective than batch chromatography.The OctaveTM MCC platform is designed to purify biomole...
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Introduction to the Multi-Column technique

MCC can be run continuously and intensifies the chromatography step, compared to batch chromatography.Compared to an injection of a distinct sample volume onto a comparably large column as done in classic batch- or single-column chromatography, MCC t...
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Multi-column chromatography (MCC)

Multi-column chromatography (MCC) is an innovative downstream processing (DSP) method for the purification of biological targets.MCC can be run continuously and intensifies the chromatography step, compared to batch chromatography. This is accomplish...
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Pre-packed Chromatography Columns

Pre-packed chromatography columns for purification are ready-to-use, available with different kinds of chromatography resins. Sizes range from milliliter scale for screening and method development up to tens of liters for GMP manufacturing. The colum...
Education Experts in Chromatography

Ion Exchange Chromatography

 Ion Exchange Chromatography (IEC or IEX) is driven by interactions between charged target molecules and charged immobilized ligands on the chromatography resin. IEX can be divided into two different sub types. Cation exchange chromatography, in...
Experts in Chromatography Education

SEC columns – the tool for size exclusion chromatography

All chromatography columns that separate mixtures of molecules by size are SEC columns. They consist of a column housing filled with porous particles that are responsible for the retention of molecules of different sizes contained in a sample and thu...
Education Experts in Chromatography

A Closer Look at Size Exclusion Chromatography (SEC) in Bioprocessing

July 16, 2025

Unleashing the Power of Size Exclusion Chromatography (SEC) in BioprocessingSize Exclusion Chromatography (SEC), also known as gel filtration chromatography, is a cornerstone technique in the biopharmaceutical industry, prized for its ability to sepa...
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