HLC-723G11 – Column & Reagents For β-Thalassemia

Hemoglobin (Hb) is a tetramer which is composed of 2 α-chains and 2 non-α-chains. In the normal synthesis, α-chains and non α-chains (β-chain, γ-chain and δ-chain) are synthesized with no overproduction or underproduction. Thalassemia is a hereditary disorder that is caused by abnormally synthesized globin chains. In thalassemic disorders, due to a quantitative defect in the synthesis of a specific chain, free α chains or single globin chains such as HbH (β4), HbBart’s (γ4) of hemoglobin could be synthesized. β-Thalassemia, in an analogous manner, refers to reduced or absent production of hemoglobin β-chains, which is partially compensated by increased production of γ- and δ-chains. Consequently, HbA2 (α2δ2) and HbF (α2γ2) are often elevated in β-Thalassemia. 

HLC-723G11 in conjunction with TSKgel G11® β-Thal. column and the following reagents provide the accurate separation of desired hemoglobin components for diagnosis of β-Thalassemia within 5.0 minutes. 

TSKgel G11® β-Thal.
The TSKgel G11 β-Thal. is intended for the quantitative percent determination of hemoglobin F and A2 and for the detection of the major hemoglobin variants, especially HbE, HbD, HbS and HbC, in whole blood samples using the HLC-723G11 β-Thalassemia Analysis Mode to aid in the diagnosis of β-Thalassemia.

G11® β-Thalassemia Elution Buffer No. 1 (S), No. 2 (S) & No. 3 (S)
G11 β-Thalassemia Elution Buffers are used to create a step gradient elution to separate and assay HbF and HbA2. The three types of G11 β-Thalassemia elution buffers contain different salt concentrations and pH, which helps in the precise separation and quantification.

HSi Hemolysis and Wash Solution
HSi Hemolysis and Wash Solution is designed for use with HLC-723G11 Analyzer. It lyses and dilutes the whole blood specimen and then injects a small volume of this specimen onto the TSKgel G11 β-Thal. column.

G11® F&A2 Calibrator Set
The G11 F&A2 Calibrator Set is intended for the calibration of the HLC-723G11 β-Thalassemia Analysis Mode. The G11 F&A2 Calibrator Set is prepared to make its value traceable to the values of WHO reference reagent. 

G11® F&A2 Control Set
The G11 F&A2 control set is intended for performing quality control assessment of HLC-723G11 β-Thalassemia Analysis Mode. These controls can be used to evaluate and monitor the precision of the performance of an assay. Two concentration levels are provided to cover the entire clinical range.

Filter Element
The filter element is designed to remove particulates from the sample after lysis and dilution and before it is introduced into the column.