TOYOPEARL HW-40F

TOYOPEARL HW-40F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-40F resin has a 5 nm pore size and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-40F resin useful for protein separations. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

The good mechanical stability of the TOYOPEARL HW-40F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-40F resin is chemically stable from pH

2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

If higher resolution is needed, the smaller particle TOYOPEARL HW-40S resin is preferred for process SEC.