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Description

TOYOPEARL HW-65C resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-65C resin has a 100 nm pore size and a 100 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-65C resin useful for protein separations of biomolecules between 40,000 and 5,000,000 Da in size.

 

The good mechanical stability of the TOYOPEARL HW-65C resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-65C resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

 

If higher resolution is needed, the smaller particle TOYOPEARL HW-65F and TOYOPEARL HW-65S resins can also be used for process SEC.

Specifications

Resin Volume
1 L, 50 L, 5 L, 500 mL, 150 mL
Product Category
Process Media
Process Step
Polishing
Mode
Size Exclusion
Applications
Virus, Other recombinant proteins, Vaccines, Peptides, Enzymes, Aggregates, Plasma purification, Proteins
Group
Specialty
Particle Size (µm)
75
Pore Size (nm)
100

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