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Description

TOYOPEARL HW-55F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-55F resin has a 50 nm pore size and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-55F resin useful for protein separations of biomolecules between 1,000 and 700,000 Da in size.

 

The good mechanical stability of the TOYOPEARL HW-55F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-55F resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

 

If higher resolution is needed, the smaller particle TOYOPEARL HW-55S resin can also be used for process SEC.

Specifications

Mode
Size Exclusion
Particle Size (µm)
45
Pore Size (nm)
50
Process Step
Polishing
Applications
Proteins, Plasma purification, Vaccines, Enzymes, Peptides, Other recombinant proteins
Group
Specialty
Product Category
Process Media
Resin Volume
5 L, 500 mL, 1 L, 50 L, 150 mL

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