TOYOPEARL SP-650S
Brand: TOYOPEARL
Manufacturers:
Description
TOYOPEARL SP-650S media is a strong cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that has been functionalized with sulfopropyl (S) strong cation exchange groups. TOYOPEARL SP-650S resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TOYOPEARL SP-650S resin has a 100 nm pore size and a 35 µm bead size.
TOYOPEARL SP-650S resin is best used for high resolution intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle TSKgel SP-5PW resins may be used. If lower resolution is desired, the larger particle TOYOPEARL SP-650M or TOYOPEARL SP-650C are recommended. As both the TOYOPEARL SP-650 and TSKgel SP-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.
TOYOPEARL SP-650S has a dynamic binding capacity for lysozyme of 50 g/L. TOYOPEARL SP-650S resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.
Specifications
| Mode | Cation Exchange |
|---|---|
| Particle Size (µm) | 35 |
| Pore Size (nm) | 100 |
| Process Step | Intermediate Purification |
| Applications | Lipoproteins, Proteins, mAb fragments, Peptides, Other recombinant proteins, Enzymes, Aggregates, Monoclonal antibodies |
| Group | Specialty |
| Product Category | Process Media |
| Resin Volume | 5 L, 50 L, 250 mL, 1 L, 25 mL |