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Description

TSKgel Chelate-5PW is based on a 100 nm pore size polymethacrylate base material covalently bonded with iminodiacetic acid (IDA) groups.  Prior to chromatography, a metal ion, such as Zn2+, Ni2+ or Cu2+, is added to form a chelate with the IDA group. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine.

Specifications

Base Material
polymer
Column Diameter (mm)
7.5, 21.5
Column Length (cm)
7.5, 15
Housing Material
Stainless Steel
Mode
Affinity
Particle Size (µm)
10
Pore Size (nm)
100
Applications
Proteins, Peptides, Enzymes
Group
Specialty
Product Category
HPLC Columns

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