The International Symposium on the Separation of Proteins, Peptides and Polynucleotides (ISPPP) is one of the premier scientific conferences dedicated to advancing separation, purification, and characterization technologies for biologically important molecules. The conference brings together leading researchers, biopharmaceutical innovators, and separation science experts to share breakthroughs in chromatography, bioanalysis, process development, and emerging biotherapeutic modalities. Through its high-level scientific presentations, workshops, posters, and technical discussions, ISPPP serves as an important forum for exchanging ideas, fostering collaboration, and driving innovation across the life sciences community. Tosoh Bioscience is excited to participate in this leading scientific event, engaging with experts from around the world and contributing to the ongoing advancement of separation and purification technologies that support the development of next-generation therapeutics. Date: October 19-20, 2026 |
| Chromatography Experts | |
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| Abbie Breton Applications Development Manager | Ashley DePace Territory Manager – New England |
Expert Insights from Tosoh Bioscience |
| Presentation |
| When | Tuesday, October 20th at 9:00 AM |
| Presenter(s) | Abbie Breton Applications Development Manager Tosoh Bioscience LLC King of Prussia, PA, USA |
| Topic | Integrated and intensified downstream processing within a continuous multi-column chromatography platform |
Achieving true continuity in downstream processing remains a key challenge for monoclonal antibody (mAb) production. Here, multi-column chromatography (MCC) is advanced beyond continuous capture to enable direct integration of a subsequent chromatographic purification step on a single system. Previous integration of downstream steps has been constrained by equipment and process limitations. The adapted multi-column system used in this work integrates two chromatographic steps in a single 8-column setup, supporting uninterrupted operation and efficient resin use. Using a high alkaline stable, fast kinetic ligand, and high dynamic binding capacity (DBC) Protein A affinity resin, we demonstrate robust mAb capture and subsequent polishing with a highly salt-tolerant cation exchange resin, advancing practical implementation of end-to-end continuous purification. In addition, a low-threshold pH step was applied into this process integration to achieve inline virus inactivation. A high degree of automation has been achieved with this setup, while the footprint has been reduced both by reducing the size of the systems and by saving on cost of buffers, thereby making downstream processing more sustainable. |
Key Highlights Visit the Tosoh Bioscience table to learn about our chromatography solutions for downstream bioprocessing, including:
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