Skip to main content
AAV Therapeutics Chromatography Solutions

Solutions for AAV Characterization

Explore our portfolio of chromatography products dedicated to purifying and analyzing next-generation AAV therapeutics.

Accelerating the Development of Viral Vector Therapeutics

Adeno-associated virus (AAV)-based therapies offer the unique ability to target specific tissues at the genetic level, enabling the treatment—and in some cases potential correction—of diseases that were previously difficult to address with conventional methods. As AAV programs advance from research to manufacturing, the ability to efficiently separate, characterize, and monitor critical quality attributes—such as empty versus full capsids—becomes essential.

Combining high-performance chromatography resin, advanced HPLC columns, and sensitive detection systems enables reliable separation and characterization of AAVs.


Empty capsids can jeopardize AAV therapeutics – Here's How


AAV Purification 
Enrich Full AAV Capsids with High-Resolution AEX

Efficiently separating empty and full capsids remains a critical step in AAV downstream processing. High selectivity and scalability are essential to ensure product quality while maintaining recovery and throughput.

TOYOPEARL GigaCap® Q-650S:
AEX polishing resin for empty vs full capsid preparative separation

› Safe and efficient choline-chloride gradient separation approach
› High recovery with strong enrichment performance


Request a TOYOPEARL GigaCap Q-650S sample

(1 mL or 5 mL pre-packed column)


AAV Analysis 
Characterize AAV Quality with Confidence

Accurate characterization of AAV critical quality attributes—such as empty/full ratio, aggregation, and size distribution—is essential for safely developing AAV therapies. Orthogonal analytical techniques provide a more complete view of AAV quality, enabling confident decisions across development and manufacturing workflows.

AEX Analysis for Empty/Full Capsid Ratio

TSKgel® Q-STAT: 
AEX-HPLC column for precise empty vs full capsid analytical separation 

› Reliable quantification of % full capsids 
› Method alignment with preparative AEX workflows


Request a 30-day evaluation of TSKgel Q-STAT

SEC-Based Size and Purity Analysis

TSKgel GMPWXL:
SEC-HPLC column for AAV size-based separation 

› Separates monomeric capsids, aggregates, and fragments
› Compatible with advanced detection methods (SEC-MALS)


Request a 30-day evaluation of TSKgel GMPWxl

Advanced Detection for Deeper Insight 

LenS3™ MALS Detector:
Multi-angle light scattering for AAV characterization

› Determines empty/full ratio and AAV titers
› Distinguishes capsids, aggregates, and impurities
 

Schedule a live LenS3 AAV module demo


Request your sample, 30-day evaluation, or a demo!

Back to top