Solutions for Oligonucleotide Purification
Explore our portfolio of chromatography resins dedicated to purifying next-generation oligonucleotide therapeutics.
Manufacturing Capacity to Meet the Demands of Industry Partners
Tosoh Bioscience’s process media portfolio enables flexible, scalable purification strategies tailored to diverse oligonucleotide modalities.
As of May 2026, Tosoh Bioscience has opened GT-4 manufacturing plant, increasing our resin manufacturing capacity by 70%! The nucleic acid-based therapy market is rapidly growing, and we are here to support our industry partners efficiently scale up their purification workflows with a process media portfolio tailored to increasingly diverse oligonucleotide modalities.
What Tosoh Bioscience Brings to the Table:
✓ Robust manufacturing of resin dedicated to oligonucleotide purification
✓ Durable, methacrylic polymer resins with high chemical and mechanical stability
✓ Complementary selectivity across AEX and HIC modes
MAKE IT RELIABLE. MAKE IT TOSOH.
Supporting the Development of Nucleic Acid-Based Medicines
Oligonucleotide therapeutics—including antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers—represent a rapidly maturing class of medicines designed to precisely modulate gene expression at the nucleic acid level. By directly targeting RNA through sequence specific interactions, these modalities enable therapeutic strategies that are not accessible with traditional small molecules or biologics, offering new paths to address genetic and metabolic diseases. Chemically synthesized oligonucleotides inherently generate closely related impurities - such as N‑1 and N+1, failure sequences, and protecting group variants - that can differ by a minor modification.
Successfully synthesizing oligonucleotides involves:
› Resolving full-length oligonucleotides from N‑1 / N+1
› Removing synthesis-related impurities and protecting groups
› Achieving high purity and recovery at preparative and manufacturing scale
Simplified Purification for Complex Therapies
Tosoh Bioscience’s portfolio of chromatography solutions is designed to meet these bioprocessing challenges. Our anion exchange and hydrophobic interaction chromatography media enable precise control over impurity clearance, thereby supporting the advancement of oligonucleotide therapeutics.
TSKgel® SuperQ‑5PW (20): Industry-leading AEX resin for oligonucleotide purification
TSKgel SuperQ‑5PW (20) resin is widely used for the purification of therapeutic oligonucleotides, including siRNA and antisense molecules. Its polymethacrylate backbone and strong AEX ligand enable high binding capacity and excellent resolution, even under high loading conditions.
Key benefits
› Maintains resolution of full‑length oligos from N‑1/N+1 impurities
› High capacity supports preparative scale purification
› Proven use in commercial oligonucleotide manufacturing
TSKgel® SuperQ‑4PW (20): Novel AEX resin optimized for short oligonucleotides
TSKgel SuperQ‑4PW (20) is engineered specifically for oligonucleotides ≤ 50 nucleotides, featuring a smaller pore size that increases surface area and binding capacity for short sequences. Studies demonstrate improved recovery and productivity compared to standard AEX media when purifying short oligos.
Key benefits
› Higher binding capacity for short oligonucleotides
› Improved recovery without altering purification strategy
› Complementary selectivity to TSKgel SuperQ‑5PW (20)
TSKgel® Phenyl‑3PW (20): Selective HIC resin for hydrophobic tag‑based oligo separations
TSKgel Phenyl‑3PW (20) is designed for hydrophobic interaction chromatography of oligonucleotides, particularly for workflows involving DMT‑protected or MMT-protected oligos. The resin enables selective binding of hydrophobic species and supports efficient removal of unprotected or failed synthesis products.
Key benefits
› High capacity HIC resin with solvent free operation
› Enables selective separation based on hydrophobicity
› Supports streamlined oligonucleotide purification workflows
Together, TSKgel SuperQ‑5PW (20), TSKgel SuperQ‑4PW (20), and TSKgel Phenyl‑3PW (20) resins provide a comprehensive toolkit to help achieve high‑purity oligonucleotides with confidence.