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Oligonucleotide Therapeutics Chromatography Solutions

Solutions for Oligonucleotide Purification

Explore our portfolio of chromatography resins dedicated to purifying next-generation oligonucleotide therapeutics.

Supporting the Development of Nucleic Acid-Based Medicines

Oligonucleotide therapeutics—including antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers—represent a rapidly maturing class of medicines designed to precisely modulate gene expression at the nucleic acid level. By directly targeting RNA through sequence specific interactions, these modalities enable therapeutic strategies that are not accessible with traditional small molecules or biologics, offering new paths to address genetic and metabolic diseases. Chemically synthesized oligonucleotides inherently generate closely related impurities - such as N‑1 and N+1, failure sequences, and protecting group variants - that can differ by a minor modification.  

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Successfully synthesizing oligonucleotides involves:

› Resolving full-length oligonucleotides from N‑1 / N+1
› Removing synthesis-related impurities and protecting groups
› Achieving high purity and recovery at preparative and manufacturing scale 

Simplified Purification for Complex Therapies

Tosoh Bioscience’s portfolio of chromatography solutions is designed to meet these bioprocessing challenges. Our anion exchange and hydrophobic interaction chromatography media enable precise control over impurity clearance, thereby supporting the advancement of oligonucleotide therapeutics. 

TSKgel® SuperQ‑5PW (20): Industry-leading AEX resin for oligonucleotide purification

TSKgel SuperQ‑5PW (20) resin is widely used for the purification of therapeutic oligonucleotides, including siRNA and antisense molecules. Its polymethacrylate backbone and strong AEX ligand enable high binding capacity and excellent resolution, even under high loading conditions.  


Key benefits

› Maintains resolution of full‑length oligos from N‑1/N+1 impurities
› High capacity supports preparative scale purification
› Proven use in commercial oligonucleotide manufacturing 

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Application Note PD09-A15P27A

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Application Note AN0127A-AN155

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TSKgel SuperQ‑4PW (20): Novel AEX resin optimized for short oligonucleotides

TSKgel SuperQ‑4PW (20) is engineered specifically for oligonucleotides ≤ 50 nucleotides, featuring a smaller pore size that increases surface area and binding capacity for short sequences. Studies demonstrate improved recovery and productivity compared to standard AEX media when purifying short oligos.


Key benefits

› Higher binding capacity for short oligonucleotides
› Improved recovery without altering purification strategy
› Complementary selectivity to TSKgel SuperQ‑5PW (20)

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TSKgel Phenyl‑3PW (20): Selective HIC resin for hydrophobic tag‑based oligo separations

TSKgel Phenyl‑3PW (20) is designed for hydrophobic interaction chromatography of oligonucleotides, particularly for workflows involving DMT‑protected or MMT-protected oligos. The resin enables selective binding of hydrophobic species and supports efficient removal of unprotected or failed synthesis products.  


Key benefits

› High capacity HIC resin with solvent free operation
› Enables selective separation based on hydrophobicity
› Supports streamlined oligonucleotide purification workflows

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Poster TP233 Phenyl 3PW

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Together, TSKgel SuperQ‑5PW (20), TSKgel SuperQ‑4PW (20), and TSKgel Phenyl‑3PW (20) resins provide a comprehensive toolkit to help achieve high‑purity oligonucleotides with confidence.

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